Web31 May 2024 · Next, root microbial community profiles of insertion mutants in scopoletin-8-hydroxylase (S8H; s8h) and in cytochrome P450 82C4 (CYP82C4; cyp82c4) were compared with wild type to determine whether any particular coumarin type is exclusively involved in the community shifts observed in f6′h1 (Fig. 3A). Web24 Mar 2024 · The At3g12900 protein can therefore be considered to be a scopoletin 8-hydroxylase (S8H) that catalyses hydroxylation at the C8 position of scopoletin. The …
Scopoletin 8-Hydroxylase-Mediated Fraxetin Production …
Web18 Nov 2024 · The secretion of Fe-mobilizing coumarins (e.g. fraxetin, esculetin and sideretin) by plant roots plays an important role in this process. Although the biochemical mechanisms leading to their biosynthesis have been well described, very little is known about their cellular and subcellular localization or their mobility within plant tissues. Web1 Mar 2024 · Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis. J. Exp. Bot. (2024) H.H. Tsai Scopoletin 8-hydroxylase-mediated fraxetin production is crucial for iron mobilization. Plant Physiol. (2024) R. Vanholme mallo ware microwave
Scopoletin 8-hydroxylase a novel enzyme involved in …
Web1 Mar 2024 · Arabidopsis roots were frozen in liquid nitrogen and ground with a pestle and mortar. A 50 mg sample of plant tissue was mixed in 500 µl of 80% methanol supplemented with 2.5 µM 4-methylumbelliferone as an internal standard. Samples were sonicated for 10 min and centrifuged for 10 min at 10000 g. WebAn essential enzyme for the biosynthesis of the major Arabidopsis coumarins, scopoletin and its derivatives, is Feruloyl-CoA 6′-Hydroxylase1 (F6′H1), which belongs to a large enzyme family of the 2-oxoglutarate and Fe2+-dependent dioxygenases. Web2 Aug 2024 · Scopoletin 8-hydroxylase (S8H) has been reported as a strong Fe-responsive gene encoding a 2-oxoglutarate-dependent dioxygenase [27, 28], and it can also catalyze … mallow arts festival