WebClathrin is the major protein component in the coat formed around pits and vesicles involved in receptor-mediated endocytosis. Clathrin forms a non-covalently bound triskelion structure composed of three heavy chains (192 kDa each) and three light chains (23-25 kDa each). Each leg of the triskelion structure contains one heavy and one light chain. WebClathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Interacts with HIP1. Interacts with DENND1A, DENND1B and DENND1C. Interacts with OCRL. Interacts with ERBB2.
Clathrin Heavy Chain/CLTC Antibody (TD.1) - SCBT
WebKnockdown of endogenous clathrin heavy chain showed that the Golgi apparatus failed to reform efficiently after BFA or 1-butanol removal. Instead, upon 1-butanol washout, it maintained a compact, tight morphology. Our results suggest that clathrin is required to reassemble fragmented Golgi elements. In addition, we show that after butanol ... The clathrin triskelion is composed of three clathrin heavy chains interacting at their C-termini, each ~190 kDa heavy chain has a ~25 kDa light chain tightly bound to it. The three heavy chains provide the structural backbone of the clathrin lattice, and the three light chains are thought to regulate the formation and disassembly of a clathrin lattice. There are two forms of clathrin light chains, designated a and b. The main clathrin heavy chain, located on chromosome 17 in human… recomended units of alcohol for men and women
The structures of natively assembled clathrin-coated vesicles
WebClathrin, a principal molecular scaffold for processes of this kind, forms a lattice-like coat on and around membranes. Its best-studied role is in classical endocytosis—the subject of … WebAll lanes : Anti-Clathrin heavy chain antibody (ab21679) at 1 µg/ml Lane 1 : HeLa whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : NIH 3T3 whole cell lysate Lane 4 : PC12 whole cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution Predicted band size: 180 kDa WebClathrin heavy chain (Chc) is a constituent of clathrin-coated vesicles and serves important functions in endocytosis and intracellular membrane trafficking but appears to … unused long service leave tax