C-terminal hemagglutinin tag
WebJun 25, 2013 · This suggests a post-translational processing of PfAtg7. We are able to attenuate levels of endogenous PfAtg7 through integration of a C-terminal regulatable fluorescent affinity (RFA) tag that allows for rapid destabilizion of … WebMolecular cloning often benefits from optimizing the vector used for expression. This pack provides our entire range of 15 mammalian N terminal tags, including epitope, affinity and solubility tags. Inserting your gene of interest into the MCS will place the tag upstream (at the N terminus of the protein), and the gene will be regulated by the ...
C-terminal hemagglutinin tag
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WebSep 13, 2024 · the C-terminal epitope of GPR126 no change in cAMP levels was observed (Supp. Fig. S1A). ... In our receptor constructs, we inserted an N-terminal hemagglutinin tag (HA-TAG, red pentagon) immediately distal to the signal peptide and a C-terminal Flag tag (FLAG-TAG, yellow pentagon) right before the Stop codon. WebNational Center for Biotechnology Information
Web您好!欢迎来到炼石商城 请登录 注册 我的订单; 我的炼石 WebAug 5, 2012 · Western blotting using an antibody against the C-terminal hemagglutinin (HA) tag of the reporter (anti-HA) or against GFP (anti-GFP) detected the 60-kDa GFP-mCherry-HA fusion protein in the...
WebTag / Fusion Protein. HA (N terminal on backbone) Growth in Bacteria. Bacterial Resistance(s) Ampicillin, 100 μg/mL Growth Temperature. 37°C ... pCDNA3- c-terminal 6xMyc pcDNA3.1-mCherry RGS-6xHis-pcDNA3.1- … WebThese mammalian expression vectors allow you to express a protein of interest fused to either the c-Myc or hemagglutinin (HA) epitope tag. c-Myc and HA are well-characterized and highly immunoreactive tags, and thus are ideal for co-IP studies but are also easily detected via Western blot.
WebAn HA-tag is derived from the human influenza hemagglutinin (HA) molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope …
WebSep 28, 2024 · Activation of GPR133 signaling with antibodies against its N-terminus To test whether GPR133 signaling is modulated by antibodies binding to the extracellular N-terminus, we transfected HEK293T cells with GPR133 tagged with an N-terminal hemagglutinin (HA) and a C-terminal FLAG epitope (Fig. 1A). how far is canberra from melbourne by carWebApr 29, 2014 · Abbreviations: CHA, C-terminus hemagglutinin tag; HA, hemagglutinin tag; HFFs, human foreskin fibroblasts; HMA, host mitochondrial association; hpi , hours postinfection; MAF1 ... To test this hypothesis, type II parasites were engineered to express MAF1 bearing a C-terminal HA epitope tag (MAF1_CHA). The HA tag served a dual … higbee\\u0027s silver grill recipesWebRecombinant Influenza A Virus Hemagglutinin H3 protein (His tag) is a HEK 293 Protein fragment 17 to 529 aa range, ... His tag C-Terminus. Additional sequence information. Influenza A Virus Hemagglutinin H3 trimeric (A/Beijing/32/92) (H3N2) protein (Genebank #: … how far is canfield ohioWebAug 19, 2024 · The H1N1 influenza pandemic vaccine has been developed from the A/California/07/09 (Cal) virus and the well-known high-yield A/Puerto Rico/8/34 (PR8) virus by classical reassortment and reverse genetics (RG) in eggs. Previous studies have suggested that Cal-derived chimeric hemagglutinin (HA) and neuraminidase (NA) … how far is cancun from ohioWebMany recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This antibody can detect either N-term or C-term HA tag. 产品编号. 产品名称. 包装. AG8057. HA Tag Rabbit Monoclonal Antibody. 50µl. AZ050. how far is cancun from mexico city by busWebAug 26, 2024 · The overexpressed GFP-tagged NT proteins were produced at an elevated level relative to the endogenous full-length protein (hemagglutinin (HA)-tagged) (Figure 1B,C). Truncation of the first 11 amino acids (NT12) appeared to slightly increase the steady state level of the expressed protein (albeit, not statistically significant), but further ... higberWebC-terminal hemagglutinin tag) (21). Cultures were preincubated with STI571 or the -secretase inhibitor L-685,458 for 2 h, then pulse labeled for 30 min with [35S]methionine [35S]cysteine Easy Tag Protein Labeling Mix (Perkin–Elmer) and chased for 2 h with inhibitors present. Media were immunoprecipitated with how far is cancun from mexico city by car